S-Allylcysteine limits cardiac structural changes via antioxidant status in ovariectomized rats with induced myocardial injury

Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main source(s): Ministry Higher Education, Malaysia (Fundamental Research Grant Scheme) Background Cardiovascular disease is the primary cause death in Malaysia. Past studies have shown incidence cardiovascular diseases among post-menopausal women increased where their estrogen level deficient. Thus, there growing interest investigating alternative approach which to limit injury for women. S-Allylcysteine (SAC) one aged garlic extract compounds that has its cardioprotective effect through mitochondrial preservation and CSE/hydrogen sulfide pathway. Purpose Hence, this study was aimed investigate SAC ovariectomized rats with isoprenaline-induced myocardial injury. Methods Ovariectomy surgery (ovary removal) done onto 32 female Wistar rats, whereas eight had same without ovary removal (Sham). After three weeks recovery, all received subcutaneous administration either normal saline (Sham, OVX) or isoprenaline 85 mg/kg (OVXIM, 2x) induce Then, oral gavage distilled water OVX, OVXIM) 100 (OVXS, OVXIMS) were given week. Finally, anesthetized before hearts cannulated on Langendorff isolated heart system cardiac function measurement. Results The rat models validated by decreased level, while from ST elevation electrocardiogram plasma Troponin T level. Systolic blood pressure OVXS (142.45±5.17 mmHg vs 120.53±6.75 mmHg) showed significant decrease after receiving SAC. Although did not reduce oxidative stress product TBARS OVXIM, but it GSH (OVX: 0.0291±0.002 vs. OVXMIS: 0.0644±0.005 nmol/mg protein) SOD 0.7148±0.039 0.4991±0.068 E/mg protein/min) antioxidants treatment group. This probably due allyl cysteine group molecule antioxidative property protects vascular tissue, thus contributes lowering pressure. Histological observation able hypertrophy collagen deposition OVXIM suggests antioxidant action may be cell stress, decreasing fibrosis (OVXIM: 5.41 ± 1.15 OVXIMS: 2.31 0.42%) preserving cardiomyocyte size 0.42 µm2). Despite histological alteration, Langendorff-perfused treated similar as Sham. CSE enzyme activity protein expression tended return Conclusion In summary, SAC’s potential structure estrogen-deficient condition, in-depth warranted further understand signalling